Żołędziewska, MagdalenaGronkiewicz, StanisławDobosz, Tadeusz2012-09-272012-09-272002Anthropological Review, vol. 65, 2002, pp. 75-800033-2003http://hdl.handle.net/10593/3467Various calcium-binding buffers and agents in DNA preparation from fresh human rib bones were compared. We showed that the buffer described by Kuntze et al. [1996] yielded the highest quantaties of DNA, but the obtained DNA was strongly degraded. The best quality DNA was obtained using two buffers: of Hänni et al. [1994] and Ivanov et al. [1995]. Among the new calcium-binding agents that were not examined before, the most efficient were Bismuth III Oxide and Na/FeEDTA, and - in two-step decalcification - sodium citrate.enDNA preparationdecalcificationDNA degradationArtykuł