Browsing by Author "Dobosz, Tadeusz"
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Item Comparison of various decalcificators in preparation of DNA from human rib bone(Polskie Towarzystwo Antropologiczne, 2002) Żołędziewska, Magdalena; Gronkiewicz, Stanisław; Dobosz, TadeuszVarious calcium-binding buffers and agents in DNA preparation from fresh human rib bones were compared. We showed that the buffer described by Kuntze et al. [1996] yielded the highest quantaties of DNA, but the obtained DNA was strongly degraded. The best quality DNA was obtained using two buffers: of Hänni et al. [1994] and Ivanov et al. [1995]. Among the new calcium-binding agents that were not examined before, the most efficient were Bismuth III Oxide and Na/FeEDTA, and - in two-step decalcification - sodium citrate.