Comparison of various decalcificators in preparation of DNA from human rib bone

Loading...
Thumbnail Image

Date

2002

Advisor

Editor

Journal Title

Journal ISSN

Volume Title

Publisher

Polskie Towarzystwo Antropologiczne

Title alternative

Abstract

Various calcium-binding buffers and agents in DNA preparation from fresh human rib bones were compared. We showed that the buffer described by Kuntze et al. [1996] yielded the highest quantaties of DNA, but the obtained DNA was strongly degraded. The best quality DNA was obtained using two buffers: of Hänni et al. [1994] and Ivanov et al. [1995]. Among the new calcium-binding agents that were not examined before, the most efficient were Bismuth III Oxide and Na/FeEDTA, and - in two-step decalcification - sodium citrate.

Description

Sponsor

Keywords

DNA preparation, decalcification, DNA degradation

Citation

Anthropological Review, vol. 65, 2002, pp. 75-80

Seria

ISBN

ISSN

0033-2003

DOI

Title Alternative

Rights Creative Commons

Creative Commons License

Uniwersytet im. Adama Mickiewicza w Poznaniu
Biblioteka Uniwersytetu im. Adama Mickiewicza w Poznaniu
Ministerstwo Nauki i Szkolnictwa Wyższego