Comparison of various decalcificators in preparation of DNA from human rib bone
| dc.contributor.author | Żołędziewska, Magdalena | |
| dc.contributor.author | Gronkiewicz, Stanisław | |
| dc.contributor.author | Dobosz, Tadeusz | |
| dc.date.accessioned | 2012-09-27T16:09:44Z | |
| dc.date.available | 2012-09-27T16:09:44Z | |
| dc.date.issued | 2002 | |
| dc.description.abstract | Various calcium-binding buffers and agents in DNA preparation from fresh human rib bones were compared. We showed that the buffer described by Kuntze et al. [1996] yielded the highest quantaties of DNA, but the obtained DNA was strongly degraded. The best quality DNA was obtained using two buffers: of Hänni et al. [1994] and Ivanov et al. [1995]. Among the new calcium-binding agents that were not examined before, the most efficient were Bismuth III Oxide and Na/FeEDTA, and - in two-step decalcification - sodium citrate. | pl_PL |
| dc.identifier.citation | Anthropological Review, vol. 65, 2002, pp. 75-80 | pl_PL |
| dc.identifier.issn | 0033-2003 | |
| dc.identifier.uri | http://hdl.handle.net/10593/3467 | |
| dc.language.iso | en | pl_PL |
| dc.publisher | Polskie Towarzystwo Antropologiczne | pl_PL |
| dc.subject | DNA preparation | pl_PL |
| dc.subject | decalcification | pl_PL |
| dc.subject | DNA degradation | pl_PL |
| dc.title | Comparison of various decalcificators in preparation of DNA from human rib bone | pl_PL |
| dc.type | Artykuł | pl_PL |