Artykuły naukowe (WB)
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Browsing Artykuły naukowe (WB) by Subject "aging"
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Item New approach to assessment of aging processes at the cellular level by NMR spectroscopy(Ośrodek Wydawnictw Naukowych Polish Academy of Sciences, 2000) Czapla, Zbigniew; Fojud, Zbigniew; Cieślik, Joachim; Jurga, StefanThe paper presents results of the proton relaxation time T1 measurements by 1H NMR for oral epithelium samples taken from 320 subjects, men and women, in different age, from 19 to 95 year old. The times of nuclear magnetic relaxation T1 were found to be correlated with the age of the subjects.Item The appraisal of the scope for the application of nuclear magnetic resonance (NMR) measurement methods for the estimation of the biological age in various stages of human ontogeny(Adam Mickiewicz University, Faculty of Biology, 1999) Czapla, Zbigniew; Fojud, ZbigniewThe main objective of the study is to find a new physical parameter meeting the criterion of the biological age estimation in various stages of human ontogeny, and thus to find a new research methodology for this field of research. For this purpose an NMR Bruker 200 MHz spectrometer was used. Two physical parameters: relaxation time (T1) and spectrum shape were determined. The research material was oral mucosa epithelium sampled in a group of young people of both sexes (31 subjects) and in a group of men (18 subjects). Relaxation time (T1) and spectrum shape were recorded for hydrogen nuclei (H1). The physical phenomena were analysed statistically (regression analysis) and their biological interpretation was attempted.Item The phaseness of human biological development assesse with the use of selected physicochemical methods(Adam Mickiewicz University Faculty of Biology, 2000) Czapla, ZbigniewThe principal idea of the work was to show a new, original method of description of the phenomena of human biological development with the use of nonstandard research methods so far unused in the ontogenetic studies in regard to the stable and involutional phases of ontogenesis. The main purpose of the work was to assess with selected methods individual reactions of a human organism in ontogenesis against the development of the population. Two research methods were selected. They were subsequently used to show individual reactions of a single human organism against the phenotypic variability characterising the population. The research methods employed are based on the physical and chemical properties of biological cells (the Electrophoretic Mobility of Cell Nuclei (EMN) method) as well as on the physical properties of selected physical parameters describing the properties of biological structures (the Nuclear Magnetic Resonance (NMR) spectroscopy method). One of the objectives of the study was to corroborate the applicability of the EMN index method as an adequate criterion 1 A summary of the thesis submitted for the degree of Doctor of Sciences in Adam Mickiewicz University, Poznañ 1999. Professor conferring the degree: Joachim Cie lik. The title of the thesis: The phaseness of human biological development assessed with the use of selected physicochemical methods . 136 Z. Czapla of biological age estimation in each phase of ontogenesis. High sensitivity of the EMN index made it possible to check to what extent a reaction of an individual, resulting from the operation of selected factors increasing the risk of premature death (nicotinism, alcoholism), would permit to assess the deviation of this individual organism from the theoretical course of its ontogenesis determined with the EMN method. Another task under the study involved checking to what degree the ontogenetic development curve in selected subject groups (alcoholics, smokers and non-smokers) determined with the EMN method deviated from the control curve. The principal research tasks regarding the methods of the Nuclear Magnetic Resonance spectroscopy involved attempts to find a new physical parameter, which could serve as a measure of the advancement of certain biological processes accompanying human ontogenesis. In other words an attempt was made to find a physical parameter which could become a new criterion of human biological age estimation regardless of the phase of ontogenesis.