Comparison of various decalcificators in preparation of DNA from human rib bone
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Date
2002
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Polskie Towarzystwo Antropologiczne
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Abstract
Various calcium-binding buffers and agents in DNA preparation from fresh human rib bones were compared. We showed that the buffer described by Kuntze et al. [1996] yielded the highest quantaties of DNA, but the obtained DNA was strongly degraded. The best quality DNA was obtained using two buffers: of Hänni et al. [1994] and Ivanov et al. [1995]. Among the new calcium-binding agents that were not examined before, the most efficient were Bismuth III Oxide and Na/FeEDTA, and - in two-step decalcification - sodium citrate.
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Keywords
DNA preparation, decalcification, DNA degradation
Citation
Anthropological Review, vol. 65, 2002, pp. 75-80