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Title: Comparison of various decalcificators in preparation of DNA from human rib bone
Authors: Żołędziewska, Magdalena
Gronkiewicz, Stanisław
Dobosz, Tadeusz
Keywords: DNA preparation
DNA degradation
Issue Date: 2002
Publisher: Polskie Towarzystwo Antropologiczne
Citation: Anthropological Review, vol. 65, 2002, pp. 75-80
Abstract: Various calcium-binding buffers and agents in DNA preparation from fresh human rib bones were compared. We showed that the buffer described by Kuntze et al. [1996] yielded the highest quantaties of DNA, but the obtained DNA was strongly degraded. The best quality DNA was obtained using two buffers: of Hänni et al. [1994] and Ivanov et al. [1995]. Among the new calcium-binding agents that were not examined before, the most efficient were Bismuth III Oxide and Na/FeEDTA, and - in two-step decalcification - sodium citrate.
ISSN: 0033-2003
Appears in Collections:Anthropological Review, 2002, vol. 65

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